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Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
Published on: September 23, 2022
Japanese encephalitis virus replicon-based vaccine expressing enterovirus-71 epitope confers dual protection from
Yi-Ting Huang1, Jia-Teh Liao2, Li-Chen Yen3
1Graduate Institute of Life Sciences, National Defense Medical Center, No. 161 Section 6, Ming Chuan E. Road, Taipei, 114, Taiwan, Republic of China (ROC). wulala902@yahoo.com.tw.
Background:
To construct safer recombinant flavivirus vaccine, we exploited Japanese encephalitis virus (JEV) replicon-based platform to generate single-round infectious particles (SRIPs) that expressed heterologous neutralizing epitope SP70 derived from enterovirus-71 (EV71). Such pseudo-infectious virus particles, named SRIP-SP70, although are not genuine viable viruses, closely mimic live virus infection to elicit immune responses within one round of viral life cycle.
Results:
We found that, besides gaining of full protection to thwart JEV lethal challenge, female outbred ICR mice, when were immunized with SRIP-SP70 by prime-boost protocol, could not only induce SP70-specific and IgG2a predominant antibodies but also provide their newborns certain degree of protection against EV71 lethal challenge.
Conclusions:
Our results therefore exemplify that this vaccination strategy could indeed confer an immunized host a dual protective immunity against subsequent lethal challenge from JEV or EV71.
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