Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Riboswitches01:56

Riboswitches

10.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
10.1K
RNA Interference01:23

RNA Interference

28.7K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.7K
RNA Interference01:23

RNA Interference

7.8K
7.8K
Experimental RNAi02:15

Experimental RNAi

8.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.3K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

1.1K
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
1.1K
Leaky Scanning02:28

Leaky Scanning

5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative genomics of Eastern-Indian <i>Ustilaginoidea virens</i> strain NRRI-FSM-1 at whole-genome level unravels genome evolution and genetic plasticity.

Frontiers in fungal biology·2026
Same author

Understanding the Evolutionary Adaptations and the Associated Functional Dynamics of Diatom <i>Cyclotella Cryptica</i>: A Chloroplast Genome-wide Comparative Study.

Current genomics·2026
Same author

Rice adaptation to low-light stress: integrating morpho-physiology, biochemical plasticity, signalling networks and molecular breeding strategies.

Plant cell reports·2026
Same author

A synthetic guide RNA scaffold enhanced CRISPR/Cas9 editing efficiency in plants across multiple gene targets.

International journal of biological macromolecules·2026
Same author

Surgical Management of Giant Cervico-Dorsal Aneurysmal Bone Cyst: Case Report with Literature-Review.

Neurology India·2026
Same author

Advances in gene editing for legume improvement: technologies, progress, and prospects.

Frontiers in genome editing·2026

Related Experiment Video

Updated: Apr 4, 2026

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
07:14

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

Published on: August 4, 2018

13.6K

Oscillating Transcriptome during Rice-Magnaporthe Interaction.

T R Sharma1, Alok Das, Shallu Thakur

  • 1ICAR-National Research Centre on Plant Biotechnology, PUSA Campus, New Delhi 110 012, India.

Current Issues in Molecular Biology
|September 14, 2015
PubMed
Summary

Understanding rice blast disease, caused by Magnaporthe oryzae, is crucial for crop protection. This review explores host-pathogen interactions using transcriptomics to enhance disease resistance and reduce rice yield losses.

More Related Videos

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

3.5K
Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone
07:36

Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone

Published on: March 17, 2023

2.4K

Related Experiment Videos

Last Updated: Apr 4, 2026

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
07:14

The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea

Published on: August 4, 2018

13.6K
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
09:25

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Published on: June 2, 2021

3.5K
Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone
07:36

Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone

Published on: March 17, 2023

2.4K

Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Genomics

Background:

  • Rice blast, caused by Magnaporthe oryzae, is a major threat to global rice production.
  • Understanding host-pathogen molecular interactions is key to developing effective disease management strategies.
  • Transcriptome analysis provides fundamental insights into gene regulation during plant-pathogen interactions.

Purpose of the Study:

  • To review recent advancements in understanding the rice-Magnaporthe oryzae pathosystem.
  • To highlight the application of transcriptomics in dissecting host-pathogen interactions.
  • To identify key determinants and potential strategies for enhancing rice disease resistance.

Main Methods:

  • Review of current literature on rice blast disease.
  • Analysis of transcriptomics approaches (e.g., RNA-Seq) applied to rice-M. oryzae interactions.
  • Examination of effector-triggered immunity (ETI) and PAMP-triggered immunity (PTI) mechanisms.

Main Results:

  • Transcriptomics has significantly advanced the understanding of molecular dialogue between rice and M. oryzae.
  • Key determinants influencing the host-pathogen interaction have been identified.
  • Insights into ETI and PTI pathways offer potential targets for breeding resistant rice varieties.

Conclusions:

  • A comprehensive understanding of rice-M. oryzae interactions through transcriptomics is vital for sustainable agriculture.
  • Identifying and utilizing resistance genes and pathways can lead to reduced crop losses.
  • Further research into host-pathogen molecular mechanisms will pave the way for novel disease control strategies.