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Chitosan polyplex nanoparticle vector for miR-145 expression in MCF-7: Optimization by design of experiment
Farnaz Sadat Mirzazadeh Tekie1, Fatemeh Atyabi2, Masoud Soleimani3
1Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, Iran.
International Journal of Biological Macromolecules
|September 15, 2015
Summary
Chitosan nanoparticles effectively deliver miR-145, a tumor suppressor, into breast cancer cells. Optimized formulation enhances gene delivery for potential cancer therapy.
Area of Science:
- Biotechnology
- Nanomedicine
- Molecular Biology
Background:
- MicroRNA-145 (miR-145) acts as a tumor suppressor, often downregulated in various cancers.
- Restoring miR-145 levels presents a therapeutic strategy for cancers like breast cancer.
Purpose of the Study:
- To develop and optimize chitosan polyplex nanoparticles for delivering miR-145 plasmid into MCF-7 breast cancer cells.
- To investigate the impact of formulation variables on nanoparticle characteristics and transfection efficiency.
Main Methods:
- Chitosan polyplex nanoparticles were fabricated using response surface methodology and D-optimal design.
- Variables studied included chitosan molecular weight (Mw) and N/P ratio.
- Nanoparticle size, zeta potential, stability, and transfection efficiency were evaluated.
Main Results:
- An interaction between Mw and N/P ratio influenced nanoparticle size.
- Formulation variables affected nanoparticle stability in serum and during storage.
- N/P ratio, incubation time, and zeta potential significantly impacted gene transfection efficiency, independent of particle size.
Conclusions:
- Optimized chitosan polyplex nanoparticle formulation is crucial for effective miR-145 delivery.
- This approach holds promise for utilizing miR-145 as an anticancer therapeutic agent.
