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Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome
Christina E Douglas1, David A Kulesh1, James G Jaissle1
1Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
Molecular and Cellular Probes
|September 15, 2015
Abstract:
Here we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).

