New insights into the FLPergic complements of parasitic nematodes: Informing deorphanisation approaches

Area of Science:

• Parasitology
• Molecular Biology
• Genomics

Background:

• FMRFamide-like peptides (FLPs) and their receptors are potential drug targets for controlling parasitic nematodes.
• Current knowledge of FLPs and FLP receptors in parasites is limited, hindering drug development.
• 31 FLP genes and 13 FLP-activated G-protein coupled receptors (GPCRs) are known in *C. elegans*.

Purpose of the Study:

• To identify and characterize FLP and FLP-GPCR gene homologues across a range of parasitic nematodes.
• To provide a comprehensive, genome-based overview of the FLP system in nematodes.
• To facilitate the discovery of novel anthelmintic targets.

Main Methods:

• Homology-based BLAST searches were employed to identify putative FLP and FLP-GPCR genes.
• Phylogenetic analyses were conducted to confirm gene relationships and conservation.
• Genome data from 17 different nematode parasite species were analyzed.

Main Results:

• Putative homologues of FLP and FLP-GPCR genes were identified in all 17 studied nematode parasites.
• This study presents the first pan-phylum, genome-based inventory of the FLP system in parasitic nematodes.
• The identified genes provide a resource for further functional studies.

Conclusions:

• The findings establish a foundation for understanding the FLP system in parasitic nematodes.
• This research paves the way for deorphanizing FLP receptors and identifying new anthelmintic drug targets.
• The study highlights the potential of FLP-GPCRs as targets for novel nematode parasite control strategies.