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Vascularized axillary lymph node transfer: A novel model in the rat.

Grzegorz J Kwiecien1, Safak Uygur1, Jason Korn1

  • 1Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH.

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|September 15, 2015
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Summary

Axillary lymph node transfer in rats is feasible using microvascular techniques. This reliable vascularized lymph node transfer (VLNT) model supports further research in lymphedema and transplantation.

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Area of Science:

  • Microsurgery
  • Transplantation Biology
  • Lymphedema Research

Background:

  • Vascularized lymph node transfer (VLNT) is a key microvascular technique for lymphedema treatment.
  • Limited experimental animal models exist to test VLNT viability.
  • Rat models are crucial for advancing surgical techniques.

Purpose of the Study:

  • To establish a feasible rat model for axillary VLNT.
  • To assess the viability of lymph nodes post-transfer.
  • To provide a reliable model for lymphedema and transplantation studies.

Main Methods:

  • Harvesting axillary lymph node flaps based on axillary vessels in Lewis rats.
  • Performing microanastomosis to femoral vessels.
  • Utilizing Indocyanine green (ICG) angiography and histology for assessment.

Main Results:

  • Successful transfer and perfusion confirmed by ICG angiography.
  • All flaps remained viable with patent vessels at 7 days post-operation.
  • Histology confirmed the presence of viable lymph nodes (3.6 ± 0.5 per flap).

Conclusions:

  • Axillary VLNT in rats is a feasible and reliable surgical procedure.
  • The model does not require donor animals and yields consistent lymph node yield.
  • This VLNT model can advance research in lymphedema, transplantation, and immune tolerance.