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Published on: October 27, 2011
Compartmentation of deoxypyrimidine nucleotides for nuclear DNA replication in S phase mammalian cells
1Department of Obstetrics and Gynaecology, University of Virginia, Charlottesville 22908.
Abstract:
DNA synthesis in S phase Chinese hamster embryo fibroblast cells in the presence of exogenous 3H-dUrd shows incorporation of the labeled precursor with very little dilution by the large unlabeled intracellular precursor pools. Full mixing would predict a specific activity 10-fold less than that measured. This coupled with the finding that 80% of the radioactivity derived from the exogenous 3H-dUrd appears in the karyoplasts implies a compartmentation where 3H-dUMP and 3H-dTTP derived from exogenous 3H-dUrd do not mix freely with endogenous cytoplasmic pools.
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