[Determination of nicotinic acid in injections by high-performance liquid chromatography]

A high-performance liquid chromatographic method for determination of nicotinic acid (NA) in commercial injections was established. NA was determined by high-performance liquid chromatography on an Inertsil ODS column (4.6 x 150 mm) using a mixture of 0.05M monobasic sodium phosphate solution (pH 3) and methanol (80:20) containing 1.5 mM sodium 1-octanesulfonate as the mobile phase at 35 degrees C and the detection wavelength at 260 nm. The working curve for 0 to 120 micrograms nicotinic acid/ml against 100 micrograms caffeine/ml as internal standard passed through the origin and was linear. On comparing the analytical data obtained by this method and those by the colorimetric method, both data were in good agreement.