[Determination of seven ultraviolet absorbent in cosmetics by high speed liquid chromatography]

A rapid method for the HPLC determination of ultraviolet absorbent (UV abs.) in cosmetics was investigated. This method is based on the technique of solvent extraction of the sample by HPLC with silica-ODS (Develosil ODS-5) as the stationary phase A, B, C, and D as the eluent and the ultraviolet spectrophometer (310, 284 nm) as the detector. The plots of the peak height vs amount of seven UV abs. were linear between 0-20 micrograms/ml. The recoveries of UV abs. to a model sample at the level of 0.1% were 93.2-100.0% by this method. This method is simple and rapid, therefor it is applicable for the determination of UV abs. in cosmetics in routine analysis.