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Lymphoid cell fractionation by aggregated immunoglobulin-agarose columns
Journal of Immunological Methods
|January 1, 1976
Summary
Aggregated immunoglobulin (Agg Ig)-agarose columns effectively separate specific immune cells from spleen cell populations. These columns isolate phagocytes and antibody-forming cells but not B or T lymphocytes, aiding in immune cell research.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Effective separation of lymphoid cell populations is crucial for immunological research.
- Existing methods may lack specificity or efficiency in isolating particular cell types.
Purpose of the Study:
- To evaluate aggregated immunoglobulin (Agg Ig)-agarose columns as a method for fractionating lymphoid cells.
- To determine which specific immune cell populations are retained by Agg Ig columns.
Main Methods:
- Fractionation of rat and mouse spleen cells using columns packed with Agg Ig-agarose.
- Analysis of retained and non-retained cell populations based on functional assays and surface markers.
Main Results:
- Agg Ig columns retained phagocytes, IgM- and IgG-antibody-forming cells, and cytotoxic T cells from rat and mouse spleen cells.
- These columns did not selectively remove B lymphocytes or T lymphocytes involved in proliferation or graft-versus-host reactivity.
Conclusions:
- Agg Ig-agarose chromatography is a valuable technique for isolating specific immune cell subsets, particularly antibody-producing cells and phagocytes.
- This method offers a means to enrich for or deplete specific cell types in immune cell studies.