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A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
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Comparative analysis of Staphylococcus epidermidis strains utilizing quantitative and cell surface shaving proteomics
Nestor Solis1, Joel A Cain2, Stuart J Cordwell3
1School of Molecular Bioscience, The University of Sydney 2006, Australia.
Journal of Proteomics
|September 16, 2015
Summary
Virulent Staphylococcus epidermidis strains form biofilms, making them hospital risks. Proteomics revealed unique surface proteins in virulent strains, including those for antibiotic resistance and CRISPR defense, suggesting potential vaccine targets.
Area of Science:
- Microbiology
- Proteomics
- Bacterial Pathogenesis
Background:
- Staphylococcus epidermidis is an opportunistic pathogen causing hospital-acquired infections.
- Virulent strains form biofilms, complicating eradication efforts.
- Understanding surface protein differences between virulent and avirulent strains is crucial.
Purpose of the Study:
- To profile and compare surface-exposed and whole-cell proteins of virulent (RP62A) and avirulent (ATCC12228) Staphylococcus epidermidis strains.
- To identify proteins associated with biofilm formation and virulence in S. epidermidis.
- To explore potential therapeutic targets, such as vaccine antigens.
Main Methods:
- Cell shaving proteomics to identify surface-exposed proteins.
- Quantitative proteomics using iTRAQ and LC-MS/MS for whole-cell proteome analysis.
- Bioinformatic analysis to identify differentially abundant and surface-localized proteins.
Main Results:
- Identified 78 surface-exposed proteins, with only 19 common to both strains.
- RP62A exhibited unique proteins involved in biofilm formation, antigenicity, and antibiotic resistance.
- 1610 whole-cell proteins were identified, with 191 differentially abundant between strains.
- Virulent RP62A showed enhanced CRISPR-mediated defense, sulfate assimilation, and antibiotic resistance.
Conclusions:
- Proteomic analysis distinguished virulent and avirulent S. epidermidis strains based on surface and whole-cell protein profiles.
- Virulent strains possess enhanced mechanisms for defense and antibiotic resistance, linked to elevated methionine levels.
- Surface proteins of virulent S. epidermidis RP62A are potential vaccine candidates for preventing implant-associated infections.
Keywords:
Cell shavingMass spectrometryMethionine biosynthesisS. epidermidisSulfate assimilationSurfaceome
