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Updated: Apr 3, 2026

Methodology for Accurate Detection of Mitochondrial DNA Methylation
Published on: May 20, 2018
An RNA-based approach to sequence the mitogenome of Hypoptopoma incognitum (Siluriformes: Loricariidae)
Daniel Andrade Moreira1, Maithê G P Magalhães1, Paula C C de Andrade1
1a Laboratório de Toxicologia Ambiental , Fundação Oswaldo Cruz (FIOCRUZ) , Rio de Janeiro , Brasil .
Abstract:
Hypoptopoma incognitum is a fish of the fifth most species-rich family of vertebrates and abundant in rivers from the Brazilian Amazon. Only two species of Loricariidae fish have their complete mitogenomes sequence deposited in the Genbank. An innovative RNA-based approach was used to assemble the complete mitogenome of H. incognitum with an average coverage depth of 5292×. The typical vertebrate mitochondrial features were found; 22 tRNA genes, two rRNA genes, 13 protein-coding genes, and a non-coding control region. Moreover, the use of this approach allowed the measurement of mtRNA expression levels, the punctuation pattern of editing, and the detection of heteroplasmies.
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