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Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

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Published on: March 16, 2010

Plastid Proteomic Analysis in Tomato Fruit Development.

Miho Suzuki¹, Sachiko Takahashi¹, Takanori Kondo¹

¹Faculty of Agriculture, Shizuoka University, Shizuoka city, Shizuoka, Japan.

|September 16, 2015

Summary

Plastid differentiation involves morphological changes before protein alterations, with key proteins like CHRC and HrBP1 decreasing in non-orange fruit varieties. These findings shed light on carotenoid accumulation and fruit color development.

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Isolation and Biophysical Study of Fruit Cuticles

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Published on: March 30, 2012

Area of Science:

Plant Biology
Molecular Biology
Biochemistry

Background:

Plastid differentiation, from chloroplast to chromoplast, is crucial for fruit color development.
Understanding the proteomic and morphological changes during this transition is key to identifying regulatory mechanisms.

Purpose of the Study:

To investigate the proteome and plastid changes during 'Micro-Tom' fruit development.
To compare these changes with other tomato varieties ('Black', 'White Beauty') to understand fruit color variation.
To elucidate the relationship between fruit color and plastid differentiation.

Main Methods:

Proteomic analysis across four developmental stages of 'Micro-Tom' fruit.
Two-dimensional gel electrophoresis (2D-PAGE) for protein comparison between 'Micro-Tom', 'Black', and 'White Beauty' varieties.
Analysis of plastid morphology and thylakoid presence.

Main Results:

Photosynthesis-related proteins persist until the orange stage in 'Micro-Tom', but thylakoids disappear.
A decrease in plastid-lipid-associated protein (CHRC) and harpin binding protein-1 (HrBP1) was observed in 'Black' and 'White Beauty' varieties.
Differences in temperature-induced lipocalin (TIL) spots were noted between 'Micro-Tom' and the other varieties, suggesting varied lipocalin functions.

Conclusions:

Plastid morphology changes precede complete protein alterations during chloroplast to chromoplast differentiation.
CHRC and HrBP1, potentially acting as lipocalins, may play roles in carotenoid accumulation and fruit color.
Lipocalin-related proteins like TIL and HrBP1 are implicated in carotenoid accumulation, fruit color, and chromoplast differentiation.