Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.2K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.2K
COP Coated Vesicles00:59

COP Coated Vesicles

18.6K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
18.6K
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

306
After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
306

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifunctional carrier based on biocompatible artificial anionic polysaccharide, cross-linked with <sup>90</sup>Y(III) ions and with a built-in antitumor agent.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2026
Same author

Water-soluble conjugates with antitumor activity from starch, phenolic antioxidant and calcium salt.

Carbohydrate research·2025
Same author

Complexes of cellulose model particles with polycations: composition, properties and cytotoxicity.

Carbohydrate research·2025
Same author

Interaction of multicomponent anionic liposomes with cationic pyridylphenylene dendrimer: Does the complex behavior depend on the liposome composition?

Biochimica et biophysica acta. Biomembranes·2021
Same author

Chitosan-based multi-liposomal complexes: Synthesis, biodegradability and cytotoxicity.

International journal of biological macromolecules·2021
Same author

The one-step synthesis of polymer-based magnetic γ-Fe<sub>2</sub>O<sub>3</sub>/carboxymethyl cellulose nanocomposites.

Carbohydrate polymers·2017
Same journal

Colloids in lubrication: Development of amphiphiles from molecular structure to tribological performance.

Advances in colloid and interface science·2026
Same journal

Engineering interfacial and network Structures in high internal phase Pickering emulsions: Mechanisms, encapsulation and release of bioactive compounds, and 3D/4D food printing applications.

Advances in colloid and interface science·2026
Same journal

Quantum dot-FRET viral biosensors: Materials, surface chemistry, and recognition architectures.

Advances in colloid and interface science·2026
Same journal

Microgels prepared by microfluidics from structural design to practical applications: Development and challenge.

Advances in colloid and interface science·2026
Same journal

Interplay of capillarity and reactivity at rock/fluid interfaces.

Advances in colloid and interface science·2026
Same journal

Plant phytohormone electrochemical sensing: From functional materials and interfaces to multiplexed sensor design.

Advances in colloid and interface science·2026
See all related articles

Related Experiment Video

Updated: Apr 3, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
11:30

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

25.6K

Multi-liposomal containers.

A A Yaroslavov1, A V Sybachin1, O V Zaborova1

  • 1M.V.Lomonosov Moscow State University, Department of Chemistry, Leninskie Gory 1-3, 119991 Moscow, Russian Federation.

Advances in Colloid and Interface Science
|September 16, 2015
PubMed
Summary
This summary is machine-generated.

This study reveals how liposomes structurally reorganize upon binding to polymer brushes, detailing lipid flip-flop and segregation. This understanding enables the creation of pH-sensitive, multi-liposomal drug delivery systems.

Keywords:
Anionic liposomeFlip-flopLateral lipid segregationMulti-liposomal complexSpherical polycationic brushpH-sensitivity

More Related Videos

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

10.7K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

4.2K

Related Experiment Videos

Last Updated: Apr 3, 2026

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
11:30

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

25.6K
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

10.7K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

4.2K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Physical Chemistry

Background:

  • Liposomes are versatile nanocarriers for drug delivery.
  • Understanding liposome-polymer interactions is crucial for developing advanced delivery systems.
  • Polycationic brushes offer unique properties for complexing liposomes.

Purpose of the Study:

  • To investigate the molecular mechanisms of liposome complexation with spherical polycationic brushes (SPBs).
  • To determine the structural integrity and lipid behavior within liposomes upon binding to SPBs.
  • To explore the development of pH-sensitive, multi-liposomal drug delivery vehicles.

Main Methods:

  • Electrophoretic mobility (EPM)
  • Dynamic light scattering (DLS)
  • Fluorescence spectroscopy
  • Conductometry
  • Differential scanning calorimetry (DSC)
  • Cryogenic transmission electron microscopy (cryo-TEM)

Main Results:

  • Liposome binding to SPBs induces anionic lipid flip-flop and lateral segregation.
  • Anionic lipid geometry dictates the maximum molar fraction (ν) for liposome integrity (ν=0.3 for CL(2-), ν=0.5 for PS(1-)).
  • The number of intact liposomes per SPB particle decreases with increasing ν.
  • Multi-liposomal complexes with high loading capacity and controlled content ratios were prepared.
  • pH-sensitive liposomes released contents upon pH reduction from 7.0 to 5.0 when complexed with SPBs.

Conclusions:

  • Liposome structural reorganization is key to successful complexation with SPBs.
  • Controlled liposome-polymer interactions enable the creation of efficient drug delivery systems.
  • The developed multi-liposomal complexes show promise for targeted and triggered drug release.