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Mesoporous CLEAs-silica composite microparticles with high activity and enhanced stability
Jiandong Cui1,2,3, Shiru Jia3, Longhao Liang1
1Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhang 050018, P R China.
Scientific Reports
|September 17, 2015
Summary
Researchers developed mesoporous enzyme-silica composite microparticles using a novel immobilization method. These particles show enhanced activity, stability, and reusability for phenylalanine ammonia lyase (PAL).
Area of Science:
- Biotechnology
- Materials Science
- Enzyme Engineering
Background:
- Enzyme immobilization is crucial for enhancing enzyme stability and reusability.
- Developing efficient methods for creating mesoporous enzyme composites remains a challenge.
- Phenylalanine ammonia lyase (PAL) has applications in various fields, but its stability is limited.
Purpose of the Study:
- To develop a novel method for creating mesoporous enzyme-silica composite microparticles.
- To enhance the activity, stability, and reusability of phenylalanine ammonia lyase (PAL).
- To investigate the structural properties contributing to the improved performance of the composite microparticles.
Main Methods:
- Co-entrapment of gelatinized starch and cross-linked enzyme aggregates (CLEAs) within biomimetic silica.
- Utilizing gelatinized starch as a pore-forming agent.
- Enzymatic removal of starch using α-amylase to create mesopores.
Main Results:
- Successfully synthesized mesoporous CLEAs-silica composite microparticles.
- The composite microparticles demonstrated significantly higher activity and stability compared to native PAL, conventional CLEAs, and PAL in biomimetic silica.
- Excellent reusability and storage stability were observed for the mesoporous composite microparticles.
Conclusions:
- The novel enzyme immobilization approach yields highly active, stable, and reusable mesoporous enzyme-silica composites.
- The unique structure, combining intra-cross-linking and a mesoporous silica shell, mitigates negative enzyme-support interactions and mass transfer limitations.
- This method offers a promising strategy for preparing various bioactive mesoporous composites with superior catalytic performance.
