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Updated: Aug 14, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants
Published on: February 21, 2015
Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification
Eftychia Dimitriadou1, Masoud Zamani Esteki2, Joris Robert Vermeesch3
1Center for Human Genetics, Laboratory for Cytogenetics and Genome Research, KU Leuven, Leuven, 3000, Belgium.
Abstract:
Whole genome amplification is required to ensure the availability of sufficient material for copy number variation analysis of a genome deriving from an individual cell. Here, we describe the protocols we use for copy number variation analysis of non-fixed single cells by array-based approaches following single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.
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