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Updated: Apr 3, 2026

Bioluminescent Bacterial Imaging In Vivo
Published on: November 4, 2012
Applications of luminous bacteria enzymes in toxicology
Valentina A Kratasyuk, Elena N Esimbekova1
1Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russia. esimbekova@yandex.ru.
Abstract:
This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
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