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Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Stereochemical analysis of glycerophospholipids by vibrational circular dichroism
Tohru Taniguchi1, Daisuke Manai1, Masataka Shibata1
1Faculty of Advanced Life Science, Frontier Research Center for Post-Genome Science and Technology, Hokkaido University , Kita 21 Nishi 11, Sapporo 001-0021, Japan.
Abstract:
The stereochemistry of glycerophospholipids (GPLs) has been of interest for its roles in the evolution of life and in their biological activity. However, because of their structural complexity, no convenient method to determine their configuration has been reported. In this work, through the first systematic application of vibrational circular dichroism (VCD) spectroscopy to various diacylated GPLs, we have revealed that their chirality can be assigned by the sign of a VCD exciton couplet generated by the interaction of two carbonyl groups. This paper also presents spectroscopic evidence for the stereochemistry of GPLs isolated from bacteria, eukaryotes, and mitochondria.
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