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Analysis of the pathogenic potential of nosocomial Pseudomonas putida strains

Matilde Fernández1, Mario Porcel2, Jesús de la Torre3

  • 1Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas Granada, Spain ; Bio-Iliberis R&D Granada, Spain.

Frontiers in Microbiology
|September 18, 2015
PubMed

Insights

Four Pseudomonas putida strains were analyzed for pathogenicity. Strain HB3267 showed significant virulence across multiple models, highlighting potential risks of this opportunistic pathogen in healthcare settings.

Area of Science:

  • Microbiology
  • Clinical Research
  • Pathogen Evolution

Background:

  • Pseudomonas putida strains are common in soil and water.
  • These bacteria can act as opportunistic human pathogens, causing hospital-acquired infections.
  • Antibiotic resistance is a growing concern in bacterial infections.

Purpose of the Study:

  • To characterize the pathogenicity and virulence of four P. putida strains isolated from hospital patients.
  • To investigate the variability in disease-causing potential among these strains.
  • To explore the potential evolution of P. putida in medical environments.

Main Methods:

  • Multilocus sequence typing was performed on four P. putida isolates (HB13667, HB8234, HB4184, HB3267).
  • Antibiotic resistance profiles were determined for the isolates.
  • Pathogenicity and virulence were assessed using ex vivo (human tissue culture) and in vivo (mammalian tissues, insect larvae) models.

Main Results:

  • Significant variation in host-damaging ability was observed among the four strains.
  • HB13667 showed no pathogenic traits.
  • HB4184 caused damage only ex vivo; HB8234 affected tissue culture and rat skin in vivo but not insect larvae.
  • Strain HB3267 demonstrated pathogenicity across all tested model systems.

Conclusions:

  • P. putida strains exhibit diverse virulence potentials.
  • Strain HB3267 represents a significant pathogenic risk, causing damage in various models.
  • Understanding strain variability is crucial for managing opportunistic infections in healthcare settings.
Keywords:
Pseudomonashospitalary strainslamininopportunistic pathogen

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