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High-Quality draft genome sequence of the Lotus spp. microsymbiont Mesorhizobium loti strain CJ3Sym

Wayne Reeve1, John Sullivan2, Clive Ronson2

  • 1Centre for Rhizobium Studies, Murdoch University, Perth, WA Australia.

Standards in Genomic Sciences
|September 19, 2015
PubMed

Insights

Researchers sequenced the genome of Mesorhizobium loti strain CJ3Sym, a bacterium important for symbiosis. This study provides valuable genomic data for understanding nitrogen fixation in legumes.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriology

Background:

  • Mesorhizobium loti strain CJ3Sym was developed through the transfer of the ICEMlSym(R7A) element from M. loti R7A to a CJ3 recipient.
  • Strain CJ3 was originally isolated from a New Zealand field site in 1994.

Observation:

  • CJ3Sym is an aerobic, Gram-negative, non-spore-forming rod.
  • The genome consists of 70 scaffolds and 71 contigs, totaling 7,563,725 base pairs.

Findings:

  • The genome contains 7,331 protein-coding genes and 70 RNA-only encoding genes.
  • This high-quality draft genome sequence is a significant resource for the scientific community.

Implications:

  • The genomic data will advance research into Mesorhizobium biology and legume symbiosis.
  • This work contributes to the Global بخteriome Project - Rhizobiales and Nitrogen-fixing Bacteria (GEBA-RNB) initiative.
Keywords:
AlphaproteobacteriaGEBA-RNBNitrogen fixationRoot-nodule bacteriaSymbiosis

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