The Influence of Single Nucleotide Polymorphism Microarray-Based Molecular Karyotype on Preimplantation Embryonic...

Area of Science:

• Reproductive Medicine
• Genetics
• Embryology

Background:

• Preimplantation genetic diagnosis (PGD) is crucial for identifying chromosomal abnormalities in embryos.
• Single nucleotide polymorphism (SNP) microarray is a key technology for molecular karyotyping in PGD.
• Understanding factors influencing embryonic development potential is vital for successful PGD outcomes.

Purpose of the Study:

• To investigate the impact of molecular karyotype, determined by SNP microarray, on embryonic development potential during PGD.
• To analyze the influence of maternal age, embryo gender, and cell number on blastocyst formation rates in PGD.

Main Methods:

• Retrospective analysis of clinical data from 119 couples undergoing PGD.
• Analysis of 929 embryos with exact diagnosis and development status.
• Utilized SNP microarray for molecular karyotyping and assessed blastocyst formation rates.

Main Results:

• Balanced molecular karyotype embryos had a significantly higher blastocyst formation rate (56.6%) compared to imbalanced embryos (24.5%).
• Maternal age and embryo gender showed no significant effect on blastocyst formation rates.
• Embryos with 6-8 cells at 68 hours post-insemination exhibited higher balanced karyotype and blastocyst formation rates.

Conclusions:

• Embryos with a balanced molecular karyotype possess a significantly higher developmental potential for blastocyst formation.
• Maternal age, embryo gender, and specific abnormal karyotype types do not significantly influence blastocyst formation.
• Embryos with 6-8 blastomeres demonstrate a favorable combination of balanced karyotype and blastocyst development potential.