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Updated: Apr 3, 2026
Detecting Migration and Infiltration of Neutrophils in Mice
Published on: February 6, 2020
Neutrophil CD64 is upregulated in RA patients with lymphoma but not in other solid cancers
Akiko Komiya1, Toshihiro Matsui2, Koichiro Horie2
1a Department of Clinical Laboratory , Sagamihara National Hospital, National Hospital Organization , Kanagawa , Japan .
Objective:
To evaluate the utility of quantifying CD64 expression on neutrophils in rheumatoid arthritis patients with malignancy, especially its diagnostic role in lymphoma.
Methods:
We used flow cytometry to quantify CD64 expression on neutrophils from patients diagnosed with malignancy during the follow-up period prior to initiating treatment.
Results:
Neutrophils from 18 patients with lymphoma expressed significantly higher levels of CD64 (9635.6 ± 2123.7 molecules/cell) than those from 32 patients with other solid cancers (carcinoma) (1250.5 ± 91.1 molecules/cell) (p < 0.001). When the cutoff value was set at 2060 molecules/cell, the sensitivity and specificity of CD64 for diagnosing lymphoma was 88.9% and 94.4%, respectively.
Conclusions:
The quantitative measurement of neutrophil CD64 by flow cytometry may be useful as a subsidiary diagnostic marker in patients with suspected lymphoma. Although neutrophil CD64 is currently a well-known marker of infection, it is necessary to bear in mind that lymphoma is also a candidate in differential diagnosis when CD64 expression on neutrophils is upregulated.
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