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Related Concept Videos

Isotopes01:12

Isotopes

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Elements have a set number of protons that determines their atomic number (Z). For example, all atoms with eight protons are oxygen; however, the number of neutrons can vary for atoms of the same element. The sum of the number of protons and the number of neutrons is the mass number (A). Atoms with the same atomic number but different mass numbers are called isotopes. Elements can have multiple isotopes, for example, carbon-12, carbon-13, and carbon-14.
An element's atomic mass, or weight,...
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Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

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Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
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Isotopes and Radioisotopes01:28

Isotopes and Radioisotopes

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In the early 1900s, English chemist Frederick Soddy realized that an element could have atoms with different masses that were chemically indistinguishable. These different types are called isotopes — atoms of the same element that differ in mass. Isotopes differ in mass because they have different numbers of neutrons but are chemically identical because they have the same number of protons. Soddy was awarded the Nobel Prize in Chemistry in 1921 for this discovery.
An isotope containing...
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Distance Corrections01:15

Distance Corrections

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To achieve precise distance measurements, especially in surveying and construction, certain corrections must be applied to account for potential sources of error like the standardization errors, temperature variations, and slope adjustments.Standardization error emerges when measurement equipment undergoes changes, such as wear, repairs, or weather impacts. To address this, surveyors compare the equipment’s readings to a standard. This process identifies any deviation that might lead to...
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NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

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When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

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An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
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Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
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ICT: isotope correction toolbox.

Christian Jungreuthmayer1, Stefan Neubauer2, Teresa Mairinger2

  • 1Austrian Centre of Industrial Biotechnology (ACIB), Vienna, Austria, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria and.

Bioinformatics (Oxford, England)
|September 19, 2015
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Summary
This summary is machine-generated.

This study introduces a new software tool to correct isotopic interferences in mass spectrometry data. The Isotope Correction Toolbox addresses challenges in analyzing biological metabolism using isotope tracer experiments.

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Area of Science:

  • Metabolomics
  • Systems Biology
  • Analytical Chemistry

Background:

  • Isotope tracer experiments are crucial for studying biological metabolism.
  • Naturally abundant isotopes and derivatization complicate mass spectrometry analysis.
  • Accurate correction of isotopic interference is numerically challenging, especially for complex systems.

Purpose of the Study:

  • To develop a freely available tool for correcting isotopic interference in tandem mass spectrometry data.
  • To provide a solution for analyzing complex isotopic systems in biological metabolism studies.
  • To enable accurate data analysis from isotope labeling experiments.

Main Methods:

  • Development of the Isotope Correction Toolbox using the Perl programming language.
  • Implementation of algorithms to correct additive interferences in isotopomer data.
  • Ensuring multi-platform compatibility for broad accessibility.

Main Results:

  • The Isotope Correction Toolbox effectively corrects isotopic interference in tandem mass spectrometry data.
  • The tool simplifies complex calculations required for isotopic interference correction.
  • It provides a solution for previously unaddressed challenges in analyzing positional information from collision-induced dissociation.

Conclusions:

  • The Isotope Correction Toolbox is a valuable, freely available resource for researchers.
  • It enhances the accuracy and reliability of isotope tracer experiments in biological systems.
  • The software supports analysis across various computer platforms, promoting wider adoption.