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Expanded test method for peptides >2 kDa employing immunoaffinity purification and LC-HRMS/MS

Andreas Thomas1, Katja Walpurgis1, Laura Tretzel1

  • 1Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933, Cologne, Germany.

Drug Testing and Analysis
|September 19, 2015
PubMed
Summary

A new method efficiently detects performance-enhancing bioactive peptides, including insulins and growth hormones, in athlete samples. This combined approach uses ultrafiltration, immunoaffinity purification, UHPLC, and mass spectrometry for comprehensive sports drug testing.

Keywords:
Doping controlsmass spectrometrysmall proteins

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