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Complete Sequence of a blaOXA-48-Harboring IncL Plasmid from an Enterobacter cloacae Clinical Isolate

Vera Manageiro1, Margarida Pinto2, Manuela Caniça3

  • 1National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AMR-HAI), Department of Infectious Diseases, National Institute of Health Dr. Ricardo Jorge, Lisbon, Portugal CECA, ICETA, Centro de Estudos de Ciência Animal, Universidade do Porto, Porto, Portugal.

Genome Announcements
|September 19, 2015
PubMed

Insights

A novel IncL plasmid, pUR17313-1, was identified in an Enterobacter cloacae clinical isolate, carrying the blaOXA-48 gene. This plasmid harbors mobile genetic elements that facilitate the spread of antibiotic resistance, including the Tn1999.2 composite transposon.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Enterobacter cloacae is a significant opportunistic pathogen.
  • Antibiotic resistance is a growing global health concern.
  • Plasmids are key vectors for the dissemination of antimicrobial resistance genes.

Purpose of the Study:

  • To characterize a novel conjugative plasmid from an Enterobacter cloacae clinical isolate.
  • To identify genes and mobile genetic elements associated with antibiotic resistance on the plasmid.

Main Methods:

  • Whole-genome sequencing of the Enterobacter cloacae clinical isolate.
  • Plasmid sequence analysis to identify genetic features and mobile genetic elements.
  • Bioinformatic analysis to determine plasmid type and gene content.

Main Results:

  • A 63,584-bp conjugative IncL plasmid, designated pUR17313-1, was identified.
  • The plasmid harbors the blaOXA-48 gene, conferring carbapenem resistance.
  • The Tn1999.2 composite transposon was found on the plasmid, containing blaOXA-48, integrase, and transposase genes, indicating its role in resistance gene mobility.

Conclusions:

  • The IncL plasmid pUR17313-1 represents a significant vehicle for the spread of the blaOXA-48 carbapenemase gene.
  • The presence of mobile genetic elements like Tn1999.2 highlights mechanisms contributing to the rapid dissemination of antibiotic resistance.
  • Understanding such plasmids is crucial for developing strategies to combat antimicrobial resistance in clinical settings.

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