Related Experiment Video
Updated: Apr 3, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Pretreatment optimization of Sorghum pioneer biomass for bioethanol production and its scale-up
Manoj Koradiya1, Srinivas Duggirala1, Devayani Tipre2
1Department of Microbiology, Biogas Research Centre, Gujarat Vidhyapith, Sadra 382424, Gujarat, India.
Abstract:
Based on one parameter at a time, saccharification of delignified sorghum biomass by 4% and 70% v/v sulfuric acid resulted in maximum 30.8 and 33.8 g% sugar production from biomass respectively. The Box Behnken Design was applied for further optimization of acid hydrolysis. As a result of the designed experiment 36.3g% sugar production was achieved when 3% v/v H2SO4 treatment given for 60 min at 180°C. The process was scaled-up to treat 2 kg of biomass. During the screening of yeast cultures, isolate C, MK-I and N were found to be potent ethanol producers from sorghum hydrolyzate. Culture MK-I was the best so used for scale up of ethanol production up to 25 L capacity, which gave a yield of 0.49 g ethanol/g sugar from hydrolyzate obtained from 2 kg of sorghum biomass.
Related Concept Videos
Bioreactor Controls-III
Scale-Up Processes
Upstream Processing
Biofuels
Methods of Medium Optimization

