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Related Experiment Video

Updated: Apr 3, 2026

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DREAM Assay for Studying Microbial Electron Transfer.

A S Vishwanathan1, Ranjan Devkota2, S Siva Sankara Sai3

  • 1Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, Puttaparthi, Andhra Pradesh, 515134, India. asvishwanathan@sssihl.edu.in.

Applied Biochemistry and Biotechnology
|September 21, 2015
PubMed
Summary
This summary is machine-generated.

The dye reduction-based electron-transfer activity monitoring (DREAM) assay uses methylene blue

Keywords:
Dye decolourizationMethylene blueMicrobial electron transferReducing power

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Area of Science:

  • Microbiology
  • Biochemistry
  • Environmental Science

Background:

  • Methylene blue's color change upon reduction quantifies reducing power.
  • Microbial electron transfer is crucial for understanding metabolic processes and environmental interactions.
  • Dissolved oxygen can interfere with electron acceptor assays.

Purpose of the Study:

  • To introduce and validate the dye reduction-based electron-transfer activity monitoring (DREAM) assay.
  • To demonstrate the assay's utility in studying microbial electron transfer from organic substrates.
  • To establish a method for assessing microbial activity based on electron transfer rates.

Main Methods:

  • Utilizing the color change of methylene blue (blue to colorless) as an indicator of reduction.
  • Monitoring the rate of electron transfer from microbes to an electron acceptor.
  • Employing sodium sulfite to mitigate the interference of dissolved oxygen.

Main Results:

  • The DREAM assay successfully monitored microbial electron transfer activity.
  • Electron transfer rates correlated with microbial activity and substrate availability.
  • The assay proved effective in overcoming dissolved oxygen interference with sodium sulfite addition.

Conclusions:

  • The DREAM assay is a viable tool for studying microbial electron transfer.
  • This method provides a metric for microbial reducing power and activity.
  • The assay has potential for broader applications in microbial research and environmental monitoring.