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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

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Published on: December 25, 2015

Two-step partial nitritation/Anammox process in granulation reactors: Start-up operation and microbial

J Dosta¹, J Vila², I Sancho¹

¹Department of Chemical Engineering, University of Barcelona, C/Martí i Franquès, No. 1, 6th Floor, 08028 Barcelona, Spain.

Journal of Environmental Management

|September 21, 2015

Summary

This study successfully treated wastewater using a two-stage Partial Nitritation (PN) and Anammox process. The PN/Anammox system effectively removed ammonium and nitrite, demonstrating efficient wastewater treatment capabilities.

Keywords:

Anammox Brocadia Granulation Microbial community structure Nitrosomonas Partial nitritation Sludge reject water

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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

Area of Science:

Environmental Microbiology
Wastewater Treatment Engineering
Biotechnology

Background:

Reject water from municipal wastewater treatment plants (WWTPs) presents a significant treatment challenge.
Conventional nitrogen removal processes can be energy-intensive and complex.
Developing efficient and cost-effective nitrogen removal strategies is crucial for sustainable water management.

Purpose of the Study:

To evaluate the efficacy of a two-stage Partial Nitritation (PN)/Anammox process for treating municipal WWTP reject water at lab-scale.
To characterize the microbial communities involved in each stage of the process.
To determine the operational stability and stoichiometry of the PN/Anammox system.

Main Methods:

Implementation of a two-stage granular SBR system for PN and Anammox processes.
Monitoring of key water quality parameters, including ammonium, nitrite, and nitrate concentrations.
Microbial community analysis using 16S rRNA gene pyrosequencing.

Main Results:

Partial Nitritation (PN) was successfully achieved, yielding an effluent with a NH4(+)-N/NO2(-)-N molar ratio near 1.0.
Effective inhibition of nitrite oxidizing bacteria (NOB) was observed in the PN stage.
Stable Anammox operation was attained with a NH4(+)-N:NO2(-)-N:NO3(-)-N stoichiometry of 1:1.25:0.14.
Microbial analysis revealed the predominance of Betaproteobacteria (Nitrosomonas) in PN and Planctomycetes (Brocadia) in Anammox.

Conclusions:

The two-stage PN/Anammox process is a viable and effective method for treating challenging reject water streams.
The microbial community structure supports the efficient functioning of both PN and Anammox stages.
Further investigation into heterotrophic biomass influence on Anammox stoichiometry is warranted.