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Understanding wax screen-printing: a novel patterning process for microfluidic cloth-based analytical devices.

Min Liu1, Chunsun Zhang1, Feifei Liu1

  • 1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.

Analytica Chimica Acta
|September 22, 2015
PubMed
Summary

This study introduces a novel wax screen-printing method for fabricating microfluidic cloth-based analytical devices (μCADs). This rapid, low-energy approach enables high-throughput preparation of μCADs for diverse analytical applications.

Keywords:
Colorimetric detectionHigh-throughputLow-cost fabricationMicrofluidic cloth-based analytical devicesSmall-batch manufacturingWax screen-printing

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic cloth-based analytical devices (μCADs) offer potential for low-cost diagnostics.
  • Existing fabrication methods for μCADs can be time-consuming and energy-intensive.

Purpose of the Study:

  • To develop a simple, rapid, and cost-effective wax screen-printing method for μCAD fabrication.
  • To establish an analytical model for wax spreading in cloth substrates.
  • To demonstrate the application of fabricated μCADs in detecting analytes in artificial urine.

Main Methods:

  • A novel wax screen-printing technique utilizing low temperatures (75°C) and rapid melting (∼5 s).
  • Development and validation of an analytical model for molten wax spreading in cloth.
  • Fabrication of arrayed μCADs on a 48-chamber device.

Main Results:

  • Optimized printing achieved minimum hydrophobic wax barrier width of 100 μm and hydrophilic channel width of 1.9 mm.
  • The analytical model accurately predicted wax spreading behavior.
  • Proof-of-concept detection of glucose and protein in artificial urine using the fabricated μCADs.

Conclusions:

  • The developed wax screen-printing method is efficient for high-throughput μCAD preparation.
  • Arrayed μCADs show promise for developing advanced sensor arrays for various analytes.
  • This approach offers a new direction for low-cost, accessible analytical devices.