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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
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Nitric oxide and the thioredoxin system: a complex interplay in redox regulation
1Department of Biochemistry, Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Biochimica Et Biophysica Acta
|September 22, 2015
Summary
Nitric oxide (NO) and thioredoxin (Trx) interact to regulate cellular redox balance. This crosstalk influences cell signaling and function, impacting cellular responses to oxidative and nitrosative stress.
Area of Science:
- Cellular redox regulation
- Molecular signaling pathways
Background:
- Nitric oxide (NO) and thioredoxin (Trx) are key players in cellular redox homeostasis.
- A complex thiol-dependent interplay exists between NO and Trx, influencing various cellular pathways.
Purpose of the Study:
- To review recent advancements in understanding the interaction and cross-regulation between NO and Trx.
- To identify outstanding questions regarding NO-Trx crosstalk in cellular function and dysfunction.
Main Methods:
- Literature review of biochemical and cellular studies.
- Analysis of NO-mediated S-nitrosylation and Trx regulatory roles.
Main Results:
- Thioredoxin (Trx) regulates S-nitrosylation, attenuating nitric oxide (NO) signaling and nitrosative stress.
- Trx can also enhance NO production by supporting NO synthase activity.
- NO reciprocally affects the redox state and activity of Trx and Trx reductase.
Conclusions:
- The intricate crosstalk between NO and Trx is crucial for cellular signaling.
- Understanding this interaction is vital for comprehending the impact of reactive oxygen and nitrogen species on cellular processes.
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