Limonoids from Munronia henryi and their anti-tobacco mosaic virus activity
Ying Yan1, Chun-Mao Yuan2, Ying-Tong Di3
1Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang 550025, People's Republic of China; The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Guiyang 550002, People's Republic of China.
Abstract:
Five new compounds (1-5), including three limonoids, one diterpenoid, and one phytosterol, along with four known limonoids (6-9), were isolated from the ethanolic extracts of whole plants of Munronia henryi. Their structures were elucidated by extensive spectroscopic analysis. In addition, the anti-tobacco mosaic virus (TMV) activities of all the isolated compounds were evaluated by the conventional half-leaf and leaf-disk methods along with Western blot analysis. Most of the tested compounds showed strong antiviral activities, with IC50 values in the range of 14.8-34.0μg/mL, compared with ningnanmycin as a positive control (IC50=44.6μg/mL), and their preliminary structure-activity relationships were also discussed.
