Related Experiment Videos
I alpha-25-dihydroxyvitamin D3 increases intracellular free calcium in murine B16 melanoma
S E Hill1, S S Bleehen, S MacNeil
1Department of Medicine, Northern General Hospital, Sheffield, U.K.
The British Journal of Dermatology
|January 1, 1989
Abstract:
Vitamin D3 and its active metabolite I alpha-25-dihydroxyvitamin D3 (I alpha-25-(OH)2D3) have been reported to play a role in melanogenesis. Physiological concentrations of I alpha-25-(OH)2D3 were found to acutely elevate intracellular free calcium (using Fura 2) in B16 primary (Io) cells. Membrane phosphoinositide turnover was unaffected by I alpha-25-(OH)2D3. The rise in intracellular free calcium was entirely dependent on extracellular calcium and was not mimicked by vitamin D3. However, in neither B16-Io nor B16-F1 melanoma cells did vitamin D3 or I alpha-25-(OH)2D3 increase melanin production.