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Updated: Apr 3, 2026

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
Published on: August 9, 2024
Proteome-wide drug and metabolite interaction mapping by thermal-stability profiling
Kilian V M Huber1, Karin M Olek1, André C Müller1
1CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria.
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Thermal stabilization of proteins after ligand binding provides an efficient means to assess the binding of small molecules to proteins. We show here that in combination with quantitative mass spectrometry, the approach allows for the systematic survey of protein engagement by cellular metabolites and drugs. We profiled the targets of the drugs methotrexate and (S)-crizotinib and the metabolite 2'3'-cGAMP in intact cells and identified the 2'3'-cGAMP cognate transmembrane receptor STING, involved in immune signaling.
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