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Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
Published on: May 2, 2025
903
Correlation between the In Vitro Functionality of Stored Platelets and the Cytosolic Esterase-Induced Fluorescence
Jiexi Wang1, Xiaoyang Yi1, Minxia Liu1
1Beijing Institute of Blood Transfusion, Academy of Military Medical Sciences, Beijing, China.
Plos One
|September 22, 2015
Summary
Cytosolic esterase-induced fluorescence intensity (CEIFI) using carboxy dimethyl fluorescein diacetate (CMFDA) can assess stored platelet function. CEIFI changes correlate strongly with platelet aggregation, hypotonic shock response, CD62P expression, and apoptosis.
Area of Science:
- Hematology
- Biotechnology
- Clinical Chemistry
Background:
- Platelet storage leads to functional decline, impacting transfusion efficacy.
- Assessing stored platelet viability and function is crucial for quality control.
- Cytosolic esterase-induced fluorescence intensity (CEIFI) is a potential indicator of platelet health.
Purpose of the Study:
- To investigate the correlation between CEIFI and stored platelet function.
- To evaluate CEIFI as a novel method for assessing platelet quality during storage.
Main Methods:
- Measurement of CEIFI using carboxy dimethyl fluorescein diacetate (CMFDA) in platelets.
- Assessment of stored platelet functionality: ADP-induced aggregation, hypotonic shock response (HSR), CD62P expression, and apoptosis.
- Correlation analysis between CEIFI and functional parameters.
Main Results:
- CEIFI significantly declined during 7-day platelet storage.
- CEIFI showed high positive correlations with ADP-induced aggregation and HSR.
- CEIFI exhibited high negative correlations with CD62P expression and platelet apoptosis.
- CEIFI strongly reflects stored platelet viability and function.
Conclusions:
- CEIFI measurement effectively indicates the functional status and viability of stored platelets.
- CEIFI offers a promising, convenient method for evaluating stored platelet quality.
- Further clinical validation is recommended for CEIFI as a routine quality assessment tool.
