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[Protection of Shenlian extracts to PM2.5 infected RAW 264.7 cell damage]
Abstract:
The aim of this research is to investigate the protection of PM2.5 infected RAW264.7 cell by traditional Chinese medicine (TCM)--Shenlian(SL) extracts and to establish the damage model. We use cell growth, cell damage and oxidative stress related markers, and inflammatory cytokines as observation index to evaluate the protection of PM2.5 infected RAW264.7 by SL extract. The results showed that 50 mg x L(-1) PM2.5 could cause cell particle deposition, inhibit the growth of cells, and significantly increase the cell supernatant of LDH, NO release quantity and intracellular reactive oxygen species (ROS) level during 4 h and 24 h. In the intervention of SL extract 50, 25, 10 mg x L(-1), the particle deposition of RAW264.7 cells, cell supernatant of LDH, NO, IL(-1) beta release, MCP-1 was significantly decreased, the SOD activity increased significantly. It shows that SL extracts of PM2.5 infected RAW264.7 cell damage has obvious protective effect, the effect may be related to the direct protection of cells, reduce oxidative stress and inflammatory injury.
Insights
Shenlian (SL) extracts protect RAW264.7 cells from particulate matter (PM2.5) induced damage. SL reduces oxidative stress and inflammation, offering a protective effect against PM2.5-induced cell injury.
Area of Science:
- Environmental Health
- Pharmacology
- Cell Biology
Context:
- Particulate Matter (PM2.5) exposure is a significant environmental health concern.
- PM2.5 induces oxidative stress and inflammation in macrophages, such as RAW264.7 cells.
- Traditional Chinese Medicine (TCM) offers potential therapeutic strategies.
Purpose:
- To investigate the protective effects of Shenlian (SL) extracts against PM2.5-induced damage in RAW264.7 cells.
- To establish a cellular damage model for PM2.5 exposure.
- To evaluate SL's impact on cell viability, oxidative stress markers, and inflammatory cytokines.
Summary:
- PM2.5 exposure (50 mg x L(-1)) caused particle deposition, inhibited cell growth, and increased LDH, NO, and ROS levels in RAW264.7 cells.
- SL extracts (50, 25, 10 mg x L(-1)) significantly reduced PM2.5-induced particle deposition, LDH, NO, IL-1β, and MCP-1 release.
- SL treatment also significantly increased SOD activity, indicating a reduction in oxidative stress.
Impact:
- SL extracts demonstrate significant protective effects against PM2.5-induced RAW264.7 cell damage.
- The protective mechanism may involve direct cellular protection, mitigation of oxidative stress, and reduction of inflammatory responses.
- This research supports the potential of TCM in addressing environmental pollutant-induced cellular damage.
