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Updated: Apr 3, 2026
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
Published on: March 31, 2021
Sensitive detection of Porcine circovirus-2 by droplet digital polymerase chain reaction
Shan Zhao1, Hua Lin1, Shijie Chen1
1College of Veterinary Medicine (Zhao, Q Yan, Wen, Hao, Z Chen, Xi) and Key Laboratory of Animal Disease and Human Health of Sichuan Province (Q Yan), Sichuan Agricultural University, Chengdu, ChinaSichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, China (Lin, S Chen, Yang, Y Yan, Sun, Hu).
Abstract:
Sensitive detection of Porcine circovirus-2 (PCV-2) is very important for surveillance of postweaning multisystemic wasting syndrome. Droplet digital polymerase chain reaction (ddPCR) is novel PCR method that can achieve high precision. Our study aimed to develop a sensitive assay utilizing ddPCR to detect PCV-2. Specificity of the assay was confirmed by the failure of amplification of DNA of other relevant viruses. The detection limit for ddPCR was 25 copies/μL, a 4-fold greater sensitivity than TaqMan real-time PCR. Both methods showed a high degree of linearity (R(2) = ~1), although TaqMan real-time PCR showed less sensitivity than ddPCR for clinical detection. Our findings indicate that ddPCR might represent a promising platform for detecting PCV-2 viral loads.
