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Updated: Apr 3, 2026

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

Published on: July 20, 2022

A pump-free membrane-controlled perfusion microfluidic platform.

Vasiliy N Goral¹, Elizabeth Tran¹, Po Ki Yuen¹

¹Science and Technology, Corning Incorporated , Corning, New York 14831, USA.

Biomicrofluidics

|September 23, 2015

Summary

This summary is machine-generated.

This study introduces a pump-free microfluidic platform for cell culture and assays. It uses a passive membrane-controlled pumping principle for efficient fluid handling without external pumps.

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Last Updated: Apr 3, 2026

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Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Perfusion cell culture and assays traditionally require complex pumping systems.
Existing microfluidic devices often necessitate external pumps for fluid manipulation.
There is a need for simpler, pump-free microfluidic solutions for various applications.

Purpose of the Study:

To develop and demonstrate a novel microfluidic platform for pump-free fluid pumping.
To adapt a passive membrane-controlled pumping principle for microfluidic applications.
To showcase the utility of this platform for perfusion cell culture and cell-based assays.

Main Methods:

The platform utilizes a passive membrane-controlled pumping principle.
Hydrostatic pressure from differing liquid levels drives fluid wicking through a porous membrane.
Devices consist of wells, micro-chambers/channels, and a porous membrane for controlled fluid flow.

Main Results:

A series of pump-free membrane-controlled microfluidic devices were successfully developed.
The platform demonstrated effective pump-free perfusion for cell culture.
The system proved suitable for various cell-based assays.

Conclusions:

The developed microfluidic platform offers a pump-free solution for perfusion-based applications.
This technology leverages passive fluid dynamics for efficient microfluidic operations.
The platform enables the benefits of microfluidics, including small sample volumes and rapid fluid exchange, without external pumps.