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    We developed a new interferometric system for imaging low polarizing samples. This system enables quantitative phase acquisition with controlled fringe visibility and single-exposure reconstruction.

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    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Microscopy

    Background:

    • Quantitative phase imaging (QPI) is crucial for label-free cell analysis.
    • Conventional QPI systems often struggle with low polarizing samples.
    • Existing methods may require complex setups or multiple exposures.

    Purpose of the Study:

    • To introduce a compact, off-axis interferometric system for QPI of low polarizing samples.
    • To enable quantitative phase acquisition with enhanced fringe visibility.
    • To demonstrate a single-exposure phase reconstruction method.

    Main Methods:

    • A spatial polarization encoder was integrated into a transmission microscope at the Fourier plane.
    • The encoder selectively preserves sample information in one polarization state while erasing it in the orthogonal state.
    • Interference fringe visibility was controlled via polarization manipulations.

    Main Results:

    • The system successfully performed quantitative phase imaging of a USAF 1951 phase test target and human red blood cells.
    • Optimal fringe visibility was achieved.
    • Single-exposure phase reconstruction was demonstrated.

    Conclusions:

    • The proposed system offers a compact and efficient solution for QPI of low polarizing samples.
    • The spatial polarization encoder effectively enables quantitative phase acquisition.
    • The method provides high-quality phase images with controlled visibility and rapid reconstruction.