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Paenibacillus faecis sp. nov., isolated from human faeces

Dominique Clermont1, Maïté Gomard1, Sylviane Hamon1

  • 1Département de Microbiologie F-75015, Institut Pasteur, Collection de l'Institut Pasteur, Paris, France.

International Journal of Systematic and Evolutionary Microbiology
|September 24, 2015
PubMed

Insights

A novel spore-forming bacterium, Paenibacillus faecis sp. nov., was identified from human feces. This Gram-positive rod exhibits unique fatty acid and menaquinone profiles, distinguishing it as a new species within the Paenibacillus genus.

Area of Science:

  • Microbiology
  • Bacteriology
  • Taxonomy

Background:

  • Human feces harbor diverse microbial communities.
  • Bacterial classification relies on phenotypic and genotypic characteristics.

Purpose of the Study:

  • To characterize and classify a novel bacterial isolate from human feces.
  • To determine the phylogenetic placement and taxonomic status of strain 656.84T.

Main Methods:

  • Isolation and cultivation of bacterial strain 656.84T from human faeces.
  • Analysis of cellular fatty acids, cell wall peptidoglycan, polar lipids, and menaquinones.
  • Determination of DNA G+C content and 16S rRNA gene sequencing.
  • DNA-DNA relatedness studies with closely related species.

Main Results:

  • Strain 656.84T is a spore-forming, rod-shaped, Gram-positive bacterium.
  • Key chemotaxonomic features include anteiso-C15:0 fatty acid, meso-diaminopimelic acid, specific polar lipid profile, and MK-7 menaquinone.
  • Phylogenetic analysis based on 16S rRNA gene sequences placed the strain within the genus Paenibacillus.
  • Low DNA-DNA relatedness values (17.3% with P. timonensis, 36.8% with P. barengoltzii) confirmed its distinct species status.

Conclusions:

  • Strain 656.84T represents a novel species within the genus Paenibacillus.
  • The proposed name for this new species is Paenibacillus faecis sp. nov.
  • The type strain is 656.84T (= DSM 23593T = CIP 101062T).

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