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Paenibacillus faecis sp. nov., isolated from human faeces
Dominique Clermont1, Maïté Gomard1, Sylviane Hamon1
1Département de Microbiologie F-75015, Institut Pasteur, Collection de l'Institut Pasteur, Paris, France.
International Journal of Systematic and Evolutionary Microbiology
|September 24, 2015
Summary
A novel spore-forming bacterium, Paenibacillus faecis sp. nov., was identified from human feces. This Gram-positive rod exhibits unique fatty acid and menaquinone profiles, distinguishing it as a new species within the Paenibacillus genus.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Human feces harbor diverse microbial communities.
- Bacterial classification relies on phenotypic and genotypic characteristics.
Purpose of the Study:
- To characterize and classify a novel bacterial isolate from human feces.
- To determine the phylogenetic placement and taxonomic status of strain 656.84T.
Main Methods:
- Isolation and cultivation of bacterial strain 656.84T from human faeces.
- Analysis of cellular fatty acids, cell wall peptidoglycan, polar lipids, and menaquinones.
- Determination of DNA G+C content and 16S rRNA gene sequencing.
- DNA-DNA relatedness studies with closely related species.
Main Results:
- Strain 656.84T is a spore-forming, rod-shaped, Gram-positive bacterium.
- Key chemotaxonomic features include anteiso-C15:0 fatty acid, meso-diaminopimelic acid, specific polar lipid profile, and MK-7 menaquinone.
- Phylogenetic analysis based on 16S rRNA gene sequences placed the strain within the genus Paenibacillus.
- Low DNA-DNA relatedness values (17.3% with P. timonensis, 36.8% with P. barengoltzii) confirmed its distinct species status.
Conclusions:
- Strain 656.84T represents a novel species within the genus Paenibacillus.
- The proposed name for this new species is Paenibacillus faecis sp. nov.
- The type strain is 656.84T (= DSM 23593T = CIP 101062T).
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