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Quantitative SERS studies by combining LOC-SERS with the standard addition method.

Evelyn Kämmer1,2,3, Konstanze Olschewski1, Stephan Stöckel1,3

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Analytical and Bioanalytical Chemistry
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Summary

This study introduces a novel quantitative surface-enhanced Raman spectroscopy (SERS) method using a lab-on-a-chip (LOC) device and the standard addition method (SAM) for accurate analyte detection.

Keywords:
Congo redLab-on-a-chipSERSStandard addition method

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Microfluidics

Background:

  • Quantitative surface-enhanced Raman spectroscopy (SERS) measurements face challenges with complex samples.
  • Interference from chemically similar molecules can affect SERS accuracy.
  • Lab-on-a-chip (LOC) systems offer potential for integrated analytical measurements.

Purpose of the Study:

  • To develop and validate a new approach for quantitative SERS analysis.
  • To demonstrate the efficacy of the standard addition method (SAM) within an LOC system.
  • To quantify analytes in the presence of interfering substances using SERS.

Main Methods:

  • Implementation of the standard addition method (SAM) within a lab-on-a-chip (LOC) device.
  • Utilizing SERS for quantitative analysis of congo red as a model analyte.
  • Testing the method's performance in the presence of methyl red, a chemically related interferent.

Main Results:

  • Successful quantification of congo red using the developed SAM-LOC-SERS approach.
  • Demonstrated ability to accurately measure the target analyte despite the presence of a chemically similar molecule.
  • Proof-of-concept achieved for direct, quantitative SERS detection in complex sample matrices.

Conclusions:

  • The developed SAM-LOC-SERS approach provides a robust method for quantitative analyte determination.
  • This technique shows significant potential for analyzing complex samples with interfering compounds.
  • The study highlights the feasibility of integrated microfluidic devices for advanced spectroscopic analysis.