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In vivo histological evaluation of fractional ablative microplasma radio frequency technology using a roller tip: an
Xiaodan Li1, Lin Fang1, Luping Huang2
1Cosmetic Laser Center, Plastic Surgery Hospital of Chinese Academic of Medical Science, Peking Union Medical College, No. 33 Ba-Da-Chu Road, Shijingshan District, Beijing, China.
Abstract:
The aim of the present study is to investigate the histological characteristics associated with microplasma radio frequency (MPRF) technology in an animal study using different treatment parameters. Two white piglets, aged 6 months, received MPRF treatment using a roller tip; the treatment site was located on the dorsal skin. Four groups of parameters were adopted regarding the performance of the treatment at four zones on the dorsum. Immediately, at 7 days and at 1, 3, and 6 months posttreatment, we observed the healing process and obtained specimens from each treatment zone. Hematoxylin and eosin and Masson stainings of histological sections were performed to assess the degree of tissue injury, the heat effect, the healing process, and neocollagenesis. Heat shock protein (HSP) was also detected using immunohistochemistry. The roller tip generated a fractional treatment, which had a general trend involving an increase in depth and width with increasing pulse energy and decreasing sliding speed. During the wound healing process, dermal neocollagenesis was stimulated, remodeled, and matured gradually. The expression of HSP47 and HPS72 was elevated in the dermis surrounding the microlesions after treatment; it peaked at 1 month posttreatment and became diffuse in the dermis. MPRF is a promising fractional skin resurfacing technique. The roller tip can be used with low risk in the entire treatment zone with rapid healing. An appropriate treatment regimen should be chosen to guarantee therapeutic efficacy and safety.
Insights
Microplasma radio frequency (MPRF) technology shows promise for fractional skin resurfacing. Histological analysis revealed controlled tissue injury, neocollagenesis, and heat shock protein expression, indicating safe and effective skin remodeling.
Area of Science:
- Dermatology
- Biomedical Engineering
- Histopathology
Background:
- Microplasma radio frequency (MPRF) technology is emerging for skin rejuvenation.
- Understanding its histological effects is crucial for optimizing treatment parameters.
Purpose of the Study:
- To investigate the histological characteristics of MPRF technology.
- To evaluate the impact of different treatment parameters on skin tissue.
- To assess the wound healing and neocollagenesis response to MPRF.
Main Methods:
- MPRF treatment was applied to piglet dorsal skin using a roller tip with varied parameters.
- Histological analysis (H&E, Masson staining) and immunohistochemistry (HSP47, HSP72) were performed at multiple time points post-treatment.
- Evaluation focused on tissue injury, thermal effects, healing, and collagen production.
Main Results:
- MPRF roller tip created fractional treatment zones, with depth and width influenced by pulse energy and sliding speed.
- Significant dermal neocollagenesis, remodeling, and maturation were observed during the healing process.
- Elevated expression of HSP47 and HSP72 peaked at 1 month post-treatment, indicating cellular response to thermal injury.
Conclusions:
- MPRF technology, particularly with a roller tip, is a safe and effective fractional skin resurfacing technique.
- The treatment stimulates a robust wound healing response with neocollagenesis.
- Careful selection of treatment parameters is essential for maximizing therapeutic efficacy and ensuring patient safety.
