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Related Experiment Video

Updated: Apr 3, 2026

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
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Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo

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Proteomics of Xenopus development.

Liangliang Sun1, Matthew M Champion1, Paul W Huber1

  • 1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.

Molecular Human Reproduction
|September 24, 2015
PubMed
Summary
This summary is machine-generated.

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This study mapped protein expression changes during early Xenopus laevis development using mass spectrometry. Nearly 40% of proteins showed dynamic expression, clustering with key developmental events.

Area of Science:

  • Developmental Biology
  • Proteomics
  • Biochemistry

Background:

  • Mass spectrometry enables detailed protein characterization.
  • Understanding embryonic development requires analyzing protein expression dynamics.

Purpose of the Study:

  • To quantify protein expression changes during early Xenopus laevis development.
  • To correlate protein expression patterns with major developmental milestones.

Main Methods:

  • Utilized isotopic labeling technology for quantitative proteomics.
  • Analyzed protein expression across six developmental stages in Xenopus laevis.
  • Applied clustering analysis to identify expression patterns.

Main Results:

  • Quantified expression of nearly 6000 proteins.
Keywords:
Xenopus laevisearly vertebrate developmentexpression clusteringiTRAQ chemistrymass spectrometryquantitative proteomics

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  • Identified significant expression changes in approximately 40% of proteins.
  • Observed six distinct protein expression clusters linked to developmental events.
  • Confirmed high protein expression consistency between embryos at the same developmental stage.

    • Conclusions:

    • Protein expression undergoes significant, stage-specific changes during early Xenopus development.
    • These dynamic protein expression patterns are tightly regulated and correlate with key developmental transitions.
    • Mass spectrometry-based proteomics provides a powerful tool for dissecting developmental processes.