Related Experiment Video
Updated: Apr 3, 2026
Human Blastocyst Biopsy and Vitrification
Published on: July 26, 2019
Successful pregnancy following the transfer of vitrified blastocyst which developed from poor quality embryos on day
Xiao-Jian Zhang1, Ye-Zhou Yang1, Li-Hua Min1
1Department of Assisted Reproductive Medical Center, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Sichuan, China.
Background:
The selection of pre-embryos for transferred is based on morphological appearance. But some poor quality cleaved embryos also can be cultured to the blastocyst stage and implanted.
Objective:
To assess the clinical pregnancy outcomes of blastocyst transfer which developed from poor quality embryos.
Materials And Methods:
A total of 109 cleaved embryos with poor quality were cultured to day 5/day 6 and 27 (24.8%) blastocysts were collected from the 15 cycles/patients undergoing conventional IVF. All the blastocysts were cooling with fast-freezing. Then the blastocysts were warmed for transfer.
Results:
All of 25 vitrified blastocysts (92.6%) survived after warming and were transferred to 15 patients. Five of the women became pregnant.
Conclusion:
Our results suggest that vitrified human day 5/day 6 blastocyst transfer which develop from poor quality embryo at day 3 can contribute to increasing cumulative pregnancy rates in assisted reproduction.
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