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Updated: Apr 3, 2026
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
Published on: December 10, 2021
Resveratrol inhibits canonical Wnt signaling in human MG-63 osteosarcoma cells
Yonggen Zou1, Jiexiang Yang2, Dianming Jiang1
1Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China.
Abstract:
In the last 30 years, the 5-year-survival rate of patients with osteosarcoma has not improved as a result of the low prevalence and large tumor heterogeneity. Therefore, the development of novel drugs for the treatment of osteosarcoma is urgently required. The present study aimed to identify potential novel drugs for the treatment of osteosarcoma, thus used β‑catenin as a target and performed high content screening. In a total of 14 botanical extracts assessed, resveratrol markedly downregulated the expression of β‑catenin and significantly inhibited MG‑63 cell proliferation. CCK‑8 assay was used to confirm the anti‑osteosarcoma effect of resveratrol and flow cytometry and western blotting were performed to analyze the underlying mechanisms of the proapoptotic effects of resveratrol. β‑catenin is a vital member of the canonical Wnt signaling pathway and, therefore, the target genes of this pathway were further analyzed. The results of this analysis demonstrated that resveratrol suppressed the MG‑63 cells by inhibiting the canonical Wnt signaling pathway.
Insights
Resveratrol, a natural compound, shows promise in treating osteosarcoma by targeting beta-catenin. This study found it inhibits cancer cell growth and promotes apoptosis, offering a potential new therapeutic avenue for this rare bone cancer.
Area of Science:
- Oncology
- Pharmacology
- Biochemistry
Background:
- Osteosarcoma survival rates have stagnated due to low prevalence and tumor heterogeneity.
- Novel therapeutic strategies are urgently needed for osteosarcoma treatment.
- Targeting key molecular pathways offers a promising approach for drug development.
Purpose of the Study:
- To identify novel drug candidates for osteosarcoma treatment.
- To investigate the potential of resveratrol as an anti-osteosarcoma agent.
- To elucidate the molecular mechanisms underlying resveratrol's effects on osteosarcoma cells.
Main Methods:
- High-content screening of botanical extracts.
- Assessment of beta-catenin expression and MG-63 cell proliferation.
- Cell viability assays (CCK-8).
- Apoptosis analysis (flow cytometry and western blotting).
Main Results:
- Resveratrol significantly downregulated beta-catenin expression in MG-63 cells.
- Resveratrol inhibited MG-63 cell proliferation.
- Resveratrol induced apoptosis in osteosarcoma cells.
- Resveratrol suppressed the canonical Wnt signaling pathway.
Conclusions:
- Resveratrol exhibits significant anti-osteosarcoma activity.
- Resveratrol's efficacy is mediated through the inhibition of the canonical Wnt signaling pathway.
- Resveratrol represents a potential therapeutic agent for osteosarcoma.
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