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Updated: Apr 3, 2026

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
Published on: June 15, 2014
Hydrogen production from sugar beet juice using an integrated biohydrogen process of dark fermentation and microbial
Bipro Ranjan Dhar1, Elsayed Elbeshbishy2, Hisham Hafez3
1Civil and Environmental Engineering, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada.
Abstract:
An integrated dark fermentation and microbial electrochemical cell (MEC) process was evaluated for hydrogen production from sugar beet juice. Different substrate to inoculum (S/X) ratios were tested for dark fermentation, and the maximum hydrogen yield was 13% of initial COD at the S/X ratio of 2 and 4 for dark fermentation. Hydrogen yield was 12% of initial COD in the MEC using fermentation liquid end products as substrate, and butyrate only accumulated in the MEC. The overall hydrogen production from the integrated biohydrogen process was 25% of initial COD (equivalent to 6 mol H2/mol hexoseadded), and the energy recovery from sugar beet juice was 57% using the combined biohydrogen.
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