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Chemostat production of pediocin SM-1 by Pediococcus pentosaceus Mees 1934
Maria Papagianni1, Daniel Sergelidis1
1Dept. of Hygiene and Technology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece.
Abstract:
Production of the bacteriocin pediocin SM-1 by Pediococcus pentosaceus Mees 1934 was investigated in pH-controlled batch and chemostat cultures using a complex medium containing glucose, sucrose or fructose. In chemostat cultures operated at 150 rpm, 30°C, 60% dissolved oxygen tension, pH 6.5, and D = 0.148 h(-1) , the pediocin titer reached 185 AU/mL representing an increase of 32% compared with batch cultures in which glucose was used as the carbon source. Pediocin biosynthesis was markedly affected by the growth rate of the producer microorganism. For all carbon sources tested, pediocin production appeared to take place only at dilution rates lower than μmax . However, only glucose supported production at the very low dilution rate of 0.05 h(-1) indicating a direct regulation of pediocin biosynthesis by the carbon source. Glucose supported higher biomass productivity and higher pediocin titers and yields compared with the other sugars used.
Insights
Glucose significantly enhances pediocin SM-1 production by Pediococcus pentosaceus. This bacteriocin yield is optimized at specific growth rates and carbon sources, with glucose proving most effective.
Area of Science:
- Microbiology
- Biotechnology
- Food Science
Background:
- Pediocin SM-1 is a bacteriocin produced by Pediococcus pentosaceus.
- Bacteriocins have potential applications as food preservatives.
- Optimizing bacteriocin production is crucial for industrial applications.
Purpose of the Study:
- To investigate the production of pediocin SM-1 by Pediococcus pentosaceus Mees 1934.
- To evaluate the impact of different carbon sources (glucose, sucrose, fructose) on pediocin production.
- To determine the effect of growth rate on pediocin biosynthesis.
Main Methods:
- pH-controlled batch and chemostat cultures were employed.
- Experiments utilized a complex medium with varying carbon sources.
- Pediocin titers were measured under different growth rates and conditions.
Main Results:
- Chemostat cultures yielded 185 AU/mL of pediocin, a 32% increase over batch cultures using glucose.
- Pediocin production was dependent on growth rate, occurring only at dilution rates below the maximum specific growth rate (μmax).
- Glucose supported higher biomass productivity, pediocin titers, and yields compared to sucrose and fructose.
Conclusions:
- Pediocin SM-1 biosynthesis is directly regulated by the carbon source, with glucose being the most effective.
- Optimizing growth rate and selecting glucose as the carbon source can significantly enhance pediocin SM-1 production.
- These findings provide valuable insights for the industrial-scale production of pediocin SM-1.