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Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization

Published on: April 3, 2014

Controllable synthesis of nucleotide complex based on pH control: a small-molecule fluorescent probe as an auxiliary

Pei Zhou¹, Chong Wang, Qi-Ming Qiu

¹Key Laboratory of Clusters Science of Ministry of Education, School of Science, Beijing Institute of Technology, Beijing 100081, P. R. China. lihui@ bit.edu.cn.

Dalton Transactions (Cambridge, England : 2003)

|September 25, 2015

Abstract:

Different CMP-bpe-M(ii) (CMP = cytidine monophosphate, bpe = bis(4-pyridyl)ethylene, M(ii) = Mn(2+) and Co(2+)) complexes are synthesized controllably based on pH control and well studied by X-ray single-crystal diffraction analysis. Interestingly, the suitable pH conditions are explored by considering the pre-organization modes of CMP, bpe, and M(2+) in aqueous solution by fluorescence spectroscopy based on acid/basic titration. The organic base bpe serves as a small-molecule fluorescent probe to indicate the changes of pre-organization modes along with the changes of the solution acidity. Furthermore, a perfect self-complementary sugar-base hydrogen bond is first reported based on the crystal structure analysis in this work, and different chiralities and SHG activities of CMP-bpe-Mn(ii) complexes obtained at different pH values are studied.

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