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Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Apr 3, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Progress in multiplex loop-mediated isothermal amplification technology.

Wen-hui Lin¹, Bing-jie Zou², Qin-xin Song³

¹1. School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009,China; 2. Department of Pharmacology, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002,China;

Yi Chuan = Hereditas

|September 25, 2015

Summary

This summary is machine-generated.

Multiplex loop-mediated isothermal amplification (LAMP) enables simultaneous nucleic acid detection. General-sensed multiplex LAMP overcomes limitations of narrow-sensed approaches, offering enhanced efficiency and broader diagnostic applications.

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

Molecular Diagnostics
Biotechnology
Isothermal Amplification

Background:

Loop-mediated isothermal amplification (LAMP) is a sensitive, rapid nucleic acid detection method with low equipment needs.
Multiplex LAMP aims to enhance diagnostic efficiency, reliability, and cost-effectiveness by detecting multiple targets simultaneously.
Conventional multiplex LAMP methods often face challenges with primer interference and biased amplification, limiting multiplexing levels.

Purpose of the Study:

To review recent advancements in multiplex loop-mediated isothermal amplification (LAMP) technology.
To differentiate between narrow-sensed and general-sensed multiplex LAMP approaches.
To highlight the principles, optimization, and applications of these multiplex LAMP technologies in diagnostics.

Main Methods:

Review of narrow-sensed multiplex LAMP, including its design and amplification optimization.
Exploration of general-sensed multiplex LAMP, emphasizing miniaturized amplification in independent compartments.
Analysis of various detection methods for LAMP products, focusing on specificity and origins.

Main Results:

Narrow-sensed multiplex LAMP methods can suffer from interference and biased amplification, limiting the number of detectable targets.
General-sensed multiplex LAMP, utilizing miniaturized parallel reactions, overcomes these limitations.
General-sensed multiplex LAMP offers benefits like reduced reagent costs, automation, and suitability for on-site detection.

Conclusions:

Multiplex LAMP technology is evolving, with general-sensed approaches offering significant advantages over narrow-sensed methods.
General-sensed multiplex LAMP holds great promise for improving the efficiency, reliability, and accessibility of molecular diagnostics.
Further research and development in multiplex LAMP will expand its applications in various diagnostic fields.