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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Apr 3, 2026

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

Constructing comprehensive venom proteome reference maps for integrative venomics.

Susann Eichberg¹, Libia Sanz², Juan J Calvete²

¹a 1 Beuth Hochschule für Technik Berlin, Luxemburger Straße 10, 13353 Berlin, Germany.

Expert Review of Proteomics

|September 25, 2015

Summary

This summary is machine-generated.

Researchers created detailed snake venom maps by separating proteins before mass spectrometry (MS) analysis. This method provides a quantitative, locus-resolved view of venom complexity, crucial for understanding adaptive traits.

Keywords:

Crotalus adamanteus mass spectrometry reverse phase-HPLC snake venom proteomics two-dimensional electrophoresis venomics

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A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Related Experiment Videos

Last Updated: Apr 3, 2026

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses

Extraction of Venom and Venom Gland Microdissections from Spiders for Proteomic and Transcriptomic Analyses

Published on: November 3, 2014

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Area of Science:

Biochemistry
Molecular Biology
Zoology

Background:

Complex adaptive traits, like snake venom, require detailed molecular understanding.
Studying venom variation necessitates temporal and spatial comparisons.

Purpose of the Study:

To demonstrate the feasibility of creating locus-resolved reference venom proteome maps.
To establish a method for qualitative and quantitative analysis of venom variation.

Main Methods:

Venom proteome fractionation and quantification using RP-HPLC, SDS-PAGE, and 2DE.
Peptide-centric MS/MS analysis.
Matching LC-MS/MS data against species-specific transcriptomic databases.

Main Results:

Identification of approximately 178-180 venom protein species.
Discovery that these proteins originate from about 48 unique transcripts.
Successful application of pre-MS decomplexation and MS/MS analysis.

Conclusions:

Comprehensive venom maps are achievable with sufficient pre-MS and MS efforts.
Separating venom decomplexation from protein identification is key for quantitative, locus-resolved insights.
This approach enhances understanding of venom evolution and function.