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Updated: Apr 3, 2026
Isolation of Small Noncoding RNAs from Human Serum
Published on: June 19, 2014
A new enzyme-free quadratic SERS signal amplification approach for circulating microRNA detection in human serum
Jing Zheng1, Dandan Ma1, Muling Shi1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China. Yangrh@pku.ed.cn.
Abstract:
In this communication, we proposed a new enzyme-free quadratic SERS signal amplification approach for the ultrasensitive detection of circulating miRNA in human serum. Combined with miRNA-triggered hybridization chain reaction and Ag(+)-mediated cascade amplification, a limit of miRNA detection as low as 0.3 fM could be achieved. More importantly, our method is suitable for the direct detection of circulating miRNAs in human serum collected from patients with different stages of chronic lymphocytic leukemia (CLL).
